More articles from Volume 34, Issue 1, 2018
An immunohistochemical analysis of angiogenic profile in T1 bladder cancer with concomitant carcinoma “in situ”
Crosstalk between NCAM/FGFR and TGF-beta signalings: an in vitro study and evaluation of human kidney biopsies
Primary sinovial sarcoma of the lung - a case report
Hyperostosis frontalis interna: case report
Proliferation marker Ki-67 in early breast cancer
Article views
Early changes in the neurogenesis in the subgranular zone of the hippocampus in 5xFAD transgenic mice model of Alzheimer s disease
Institute of Histology and Embryology Aleksandar S. Kostic
School of Medicine, University of Belgrade , Belgrade , Serbia
Department of Neurobiology, Institute for Biological Research “Sini a Stankovic”
University of Belgrade , Belgrade , Serbia
Department of Neurobiology, Institute for Biological Research “Sini a Stankovic”
University of Belgrade , Belgrade , Serbia
Laboratory for Human Molecular Genetics, Institute of Molecular Genetics and Genetic Engineering
University of Belgrade , Belgrade , Serbia
Laboratory for Human Molecular Genetics, Institute of Molecular Genetics and Genetic Engineering, University of Belgrade , Belgrade , Serbia
Faculty of Biology, University of Belgrade , Belgrade , Serbia
Serbian Academy of Sciences and Arts , Belgrade , Serbia
Department of Neurobiology, Institute for Biological Research “Sini a Stankovic”, University of Belgrade , Belgrade , Serbia
Institute of Histology and Embryology Aleksandar S. Kostic
School of Medicine, University of Belgrade , Belgrade , Serbia
Published: 01.04.2018.
Volume 34, Issue 1 (2018)
pp. 13-14;
Abstract
Aim: The aim of this study was to investigate the expression of Sox1 and Sox2 transcriptional factors in the subgranular zone (SGZ) of the hippocampus in 8 weeks old 5xFAD mice. Introduction: Transgenic 5xFAD mice represent a model of Alzheimer s disease (AD) characterized by an early deposition of amyloid plaques which disrupt the process of adult neurogenesis in the hippocampus. Certain transcriptional factors such as SoxB1 transcriptional factors are involved in the process of adult neurogenesis, but their roles in neurodegenerative disorders are not fully understood. Material and Methods: Transgenic mice of both genders and their respective non-transgenic controls (n=6 per group) were used in the study. Proliferating cells and immature neurons were detected by their immunohistochemical expression of Ki67 and doublecortin, while neuronal stem/precursor cells were identified by the expression of Sox1 and Sox2 proteins. Immunohistochemistry and counting were performed on hippocampal paraffin sections. Results: Immunohistochemical analysis showed that 5xFAD mice in the SGZ of the hippocampus have significantly lower numbers of Sox1 immunoreactive cells, while Sox2 immunoreactive cells were lower only in female 5xFAD mice. Furthermore, we have detected a decrease in the number of newly formed neurons in male transgenic mice, while the number of proliferating cells was unchanged when compared to non-transgenic controls. Conclusion: The results of our study show that early changes in the neurogenesis in 5xFAD model occur despite the preserved proliferative potential in the SGZ. Our results clearly indicate the importance of SoxB1 transcriptional factors in the early phases of AD.
Keywords
Citation
Copyright
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Article metrics
The statements, opinions and data contained in the journal are solely those of the individual authors and contributors and not of the publisher and the editor(s). We stay neutral with regard to jurisdictional claims in published maps and institutional affiliations.
