The aim of our study was to determine using immunohistochemical method profi and relationship between lymphocytes in lymphoid rich stroma of the Warhins tumors, as well as, the relationship between lymphocytes and tumors epithelial cells. Our retrospective-prospective study we conducted in the Department of Clinical Pathology KBC Zemun on 25 samples of tissue from the parotid gland with WT tumor surgically removed at the Department of ENT KBC Zemun in the period from 2000 to 2016 year. Sections of tumor tissue, fixed in 10% formalin were stained with hematoxylin-eosin standard method, and then immunohistochemical method to: antibody B lymphocytes (CD20) antibody for T lymphocytes (CD3), keratin antibody that marks the basal and columnar epithelial cells (CK7 ) and keratin antibody that marks only the basal epithelial cells (CK5 / 6). We have found that in relation to the gender distribution of all 25 patients, there were more male patients: 16 (64%) men and 9 (36%) women. Immunohistochemical analysis with keratin antibody CK7 showed that luminal columnar cells intensely positive (+++) more than 75% in 23 cases (92%), whereas in 2 cases were intensely positive (+++) in percentages ranging from 25 to 75%. In contrast to the luminal, basal cells were always showed little immunoreactivity (+) antibody to CK7 and in 20 cases in the group of <25% of the cells respectively in the cases of 5% in the group 25-75. Evaluation of the presence, intensity, type and distribution of cells with each other and in relation to the tumor epithelial component would contribute to establishing unclear histogenesis lymphoid stroma at the tumor.

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.