The 20th century was the most eventful period in the history of Zemun Hospital and it brought many changes. Working through out both world wars, the hospital staff aided those who were wounded or ill, both soldiers and civilians. Throughout this period, the hospital worked in three different countries, under various administrations and owners.

Na početku 21. veka, u vremenu društveno-političkih i ekonomskih previranja, u tadašnjoj Jugoslaviji nije bilo organizovanih naučnih i edukativnih aktivnosti u oblasti ehokardiografije. Zahvaljujući entuzijazmu nekoliko srpskih kardiologa, koji su prepoznali potrebu za promenom, aprila 2001. godine u Beogradu je osnovano ehokardiografsko udruženje, sa ciljem da postavi standarde ehokardiografskog pregleda, podrži naučne aktivnosti u oblasti ehokardiografije i osnaži saradnju sa međunarodnim ehokardiografskim organizacijama. Nakon raspada bivše Jugoslavije, prvobitan naziv «Jugoslovensko ehokardiografsko udruženje» (YUECHO), 2006. godine promenjen je u Ehokardiografsko udruženje Srbije (ECHOS).

Aim: The aim of this study was to investigate the expression of Sox1 and Sox2 transcriptional factors in the subgranular zone (SGZ) of the hippocampus in 8 weeks old 5xFAD mice. Introduction: Transgenic 5xFAD mice represent a model of Alzheimer s disease (AD) characterized by an early deposition of amyloid plaques which disrupt the process of adult neurogenesis in the hippocampus. Certain transcriptional factors such as SoxB1 transcriptional factors are involved in the process of adult neurogenesis, but their roles in neurodegenerative disorders are not fully understood. Material and Methods: Transgenic mice of both genders and their respective non-transgenic controls (n=6 per group) were used in the study. Proliferating cells and immature neurons were detected by their immunohistochemical expression of Ki67 and doublecortin, while neuronal stem/precursor cells were identified by the expression of Sox1 and Sox2 proteins. Immunohistochemistry and counting were performed on hippocampal paraffin sections. Results: Immunohistochemical analysis showed that 5xFAD mice in the SGZ of the hippocampus have significantly lower numbers of Sox1 immunoreactive cells, while Sox2 immunoreactive cells were lower only in female 5xFAD mice. Furthermore, we have detected a decrease in the number of newly formed neurons in male transgenic mice, while the number of proliferating cells was unchanged when compared to non-transgenic controls. Conclusion: The results of our study show that early changes in the neurogenesis in 5xFAD model occur despite the preserved proliferative potential in the SGZ. Our results clearly indicate the importance of SoxB1 transcriptional factors in the early phases of AD.

Aim: The aim of this study was to investigate the association of local tumor survivin expression and serum concentration with clinical and histopathological parameters in melanoma patients. Introduction: Survivin is a multifunctional protein abundantly expressed in tumors of various types, including melanoma. There are still sparse data regarding relationship of melanoma cell survivin expression with accepted histopathological characteristics as well as serum concentration. Material and Methods: The level of survivin expression was determined immunohistochemically in tumor tissue and with ELISA test in the serum of 84 melanoma patients with melanoma. Results: Survivin expression was significantly higher in the patients whose tumor had ulceration, higher mitotic index, higher Clark and Breslow stage, that made vascular invasion or spread through lymphatic vessels in primary tumor, and in the patients with metastatic disease. The patients with high survivin expression score had almost double shorter disease free interval DFI comparing to those with weak local survivin expression and a small number of survivin cells (9 - 7 vs 19 - 13 months, respectively). The degree of tumor infiltrating lymphocytes presence in tumor tissue was significantly inversely associated with serum survivin concentration. Conclusion: Conclusion Survivin expression in tumor tissue and its serum concetration significantly correlate with clinical and histopathological parameters. Serum levels could be important in initial follow-up as indicators of those patients that would have aggressive local tumor growth and spreading.

Aim: The aim of this research is to analyze the profile of Ezh2 expression in superficial urothelial bladder cancer, to investigate its correlation with clinicopathological parameters, as well as to determine the prognostic significance of Ezh2. Introduction: Superficial urothelial bladder cancer, without invasion of muscle layer, is associated with frequent recurrence, and represents significant burden for health care system. Ezh2 is epigenetic regulator with a major role in urothelial oncogenesis. Clinical investigations of Ezh2 inhibitors in treatment of solid cancers have already given encouraging results. Materials and Methods: Tumor samples from 410 patients with superficial bladder cancer (172 pTa, 238 pT1), obtained by transurethral resection, were incorporated in tissue microarrays, and analyzed immunohistochemically to Ezh2 expression. Correlation analysis with clinicopathological parameters was performed using SPSS 18.0. Results: High nuclear expression was found in 33.4% tumors, and it was significantly more frequent in pT1 (46.6%), compared to pTa tumors (15.1%) (p<0.001). Ezh2 expression was associated with high histologic grade, presence of carcinoma in situ, and cancer specific death (p<0.001, respectively). In Kaplan-Mayer survival analysis high Ezh2 expression was significantly associated with poor prognosis and shorter patients survival (p<0.001). There was no significant correlation between Ezh2 and recurrence of the disease, and recurrence free interval (p<0.05). Conclusion: Immunohistochemical expression of transcription repressor Ezh2 in superficial urothelial bladder cancer indicates aggressive behavior of the tumor, and poor prognosis. Ezh2 could be used as pronostic marker in selection of the patients that might require more intense clinical treatment, and as potential target of anticancer therapy.