The aim of the research was to determine the profile of employed nurses involved in the care of patients receiving chemotherapy and to assess the level of specific education for this type of care. Specialized education, preparation and training are required for oncology nurses who administer chemotherapy and other dangerous anti-cancer drugs to ensure a safe level of care. An observational survey of the survey type was applied, and the results were presented by descriptive statistical methods. The study sample consisted of 20 respondents, nurses and technicians from the Institute of Oncology and Radiology in Belgrade. The research was conducted in the period from May 1 to 20, 2019, at the Institute of Oncology and Radiology in Belgrade. The profile of nurses in our study was that most nurses are traditionally female, with an average age of 36-55 with a high school diploma. Nurses have sufficient knowledge about malignant diseases (65%), 80% are well aware of the side effects of chemotherapy, 55% of nurses provide psychological support to patients with malignant diseases, and 75% assess pain in patients. A slightly higher percentage of 65% of nurses who devote enough time to acquainting patients with their disease and the purpose of treatment. Half of the respondents go to trainings regularly. Based on the results of the research, we can conclude that most nurses have sufficient knowledge about malignant diseases, but the number of those who need additional education is not negligible, to which additional attention must be paid.